Mokhoa oa ho etsa phofo e omisitsoeng ea lumbrokinase

Ⅰ.Tšebeliso ea Theknoloji ea ho Omisa ka Leqhoa ho Lokisetsa Earthworm Fibrinolytic Enzyme Lyophilized Powder.

Ho omisa ka leqhoa, ho tsejoang hape e le lyophilization, ke ts'ebetso e tlosang mongobo linthong tse sebelisang lisebelisoa tse omisang serame ho boloka sebopeho le ts'ebetso ea tsona. Ho lokisetsa phofo ea earthworm fibrinolytic enzyme lyophilized ho kenyelletsa mehato e 'meli e meholo: leqhoa le ho omisa ka vacuum. Ts'ebetso ena ka katleho e tlosa mongobo ho tsoa ho enzyme e nkiloeng ha e ntse e boloka ts'ebetso ea eona ea tlhaho le botsitso.

Ⅱ.Ho Hlaolela Mathata a Boleng bo Phahameng

Ho khetha mefuta e phahameng ea lihlahisoa bakeng sa tlhahiso ea li-enzyme ho bohlokoa molemong oa katleho ea ts'ebetso ea tlhahiso. Ka tlhahlobo le ntlafatso, mefuta e nang le ts'ebetso e phahameng le tlhahiso e lengoa ho ntlafatsa bokhoni ba tlhahiso ea enzyme.

Ⅲ.Ho ntšoa le Tlhoekiso

Ho sebelisoa theknoloji e tsoetseng pele ea ho ntša enzyme ho arola enzyme ea earthworm fibrinolytic ho tsoa mefuteng, ebe e lateloa ke tlhoekiso ho tlosa litšila. Mekhoa e tloaelehileng e kenyelletsa tšitiso ea ultrasonic, centrifugation, le column chromatography, e fanang ka tlhahiso ea enzyme e phahameng ea bohloeki.

Ⅳ.Mokhoa oa ho omisa ka leqhoa

Ho sebelisa"BABELI"FreezeDryerlisebelisoa, tlhahiso ea enzyme e sebetsoa hore e be phofo e tsitsitseng ea lyophilized. Nakong ea ts'ebetso ea ho omisa, liparamente tse kang mocheso le maemo a vacuum lia fetoloa ho netefatsa hore ts'ebetso ea enzyme le botsitso lia bolokoa.

Ⅴ.Melemo ea Theknoloji ea Freeze-Dryer

Theknoloji ea "bobeli" ea ho omisa e fana ka melemo e latelang ho ntlafatsa botsitso le bioavailability ea earthworm fibrinolytic enzyme lyophilized powder:

Ⅵ.Theknoloji e tsoetseng pele ea leqhoa

Theknoloji e sa tsoa etsoa -50 ° C ea leqhoa e potlakisa ho hoama ka potlako ho ntša enzyme, ho thibela ho thehoa ha kristale le ho fetoha ha mocheso ho ka senyang ts'ebetso ea enzyme.

Ⅶ.Taolo e nepahetseng ea ho omisa

"BOTH" se omisa sehatsetsi se na le sistimi e nepahetseng ea taolo ea ho omisa, e lumellang mocheso (-50 ° C ho ea + 45 ° C) le liphetoho tsa vacuum (1-30 Pa) ho latela litlhoko tsa ts'ebetso. Sena se tiisa botsitso le tšebetso ea enzyme nakong ea ho omisa.

Ⅷ.Tlhahlobo ea Liphello tsa Phokotso ea LDL-C

Enzyme ea Lyophilized earthworm fibrinolytic e sebetsitsoeng ka theknoloji ea ho omisa e boloka ts'ebetso e phahameng ea tlhaho. Liteko tsa bongaka li bontša hore phello ea eona ea ho theola LDL-C e feta haholo ea li-enzyme tse sebelisoang ka tloaelo. Lintlha tsa bohlokoa li kenyelletsa:

1.Enhanced Sollubility: Phofo ea lyophilized e qhibiliha ka potlako metsing, e lokolla likarolo tse sebetsang le ho ntlafatsa ho monya le ho sebelisoa 'meleng.

2.Paballo ya Tshebetso: Ts'ebetso ea ho omisa ka leqhoa e fokotsa ho se sebetse ha enzyme, ho e lumella ho sebetsa nako e telele 'meleng le ho ntlafatsa katleho ea ho fokotsa LDL-C.

Tšebeliso ea thekenoloji ea ho omisa ka leqhoa ha ho lokisetsa earthworm fibrinolytic enzyme lyophilized powder e boloka mosebetsi oa enzyme le botsitso ha e ntse e ntlafatsa bioavailability. Sena se fana ka tlhahiso e sebetsang ea lithethefatsi bakeng sa ho theola LDL-C le ho ntlafatsa bophelo bo botle ba methapo. Ka tsoelo-pele e tsoelang pele ea theknoloji ea ho omisa ka leqhoa, tšebeliso e atileng ea "BOTH" ea li-dryer ka tlhahiso ea phofo ea enzyme e fana ka tharollo e ncha bakeng sa thibelo le kalafo ea lefu la pelo le methapo.

Haeba u thahasella ronaMochini oa ho Omisakapa u na le lipotso, ka kopo ikutloe u lokolohile hoIteanye le rona. Joaloka moetsi ea hloahloa oa mochini o omisang sehatsetsi, re fana ka litlhaloso tse fapaneng, ho kenyeletsoa mefuta ea malapeng, ea laboratori, ea lifofane le ea tlhahiso. Hore na o hloka lisebelisoa tsa tšebeliso ea lapeng kapa lisebelisoa tse kholo tsa indasteri, re ka u fa lihlahisoa le lits'ebeletso tsa boleng bo holimo.